IDEALLY please select a sequence from below
AT plasmid complete sequence
Aeropyrum pernix K1 complete genome
Agrobacterium tumefaciens strain C58 circular chromosome, complete sequence
Agrobacterium tumefaciens strain C58 linear chromosome, complete sequence
Agrobacterium tumefaciens strain C58 plasmid AT, complete genome
Agrobacterium tumefaciens strain C58 plasmid Ti, complete genome
Agrobacterium tumefaciens strain C58, complete genome
Aquifex aeolicus complete genome
Aquifex aeolicus plasmid ece1, complete plasmid sequence
Archaeoglobus fulgidus complete genome
Bacillus anthracis str. Ames complete genome
Bacillus cereus ATCC 14579 complete genome
Bacillus cereus ATCC 14579 plasmid pBClin15, complete sequence
Bacillus halodurans C-125, complete genome
Bacillus subtilis complete genome
Bacteroides thetaiotaomicron VPI-5482, complete genome
Bifidobacterium longum NCC2705 complete genome
Blochmannia floridanus complete genome
Bordetella bronchiseptica strain RB50, complete genome
Bordetella parapertussis strain 12822, complete genome
Bordetella pertussis strain Tohama I, complete genome
Borrelia burgdorferi plasmid cp26, complete plasmid sequence
Borrelia burgdorferi plasmid cp32-1, complete plasmid sequence
Borrelia burgdorferi plasmid cp32-3, complete plasmid sequence
Borrelia burgdorferi plasmid cp32-4, complete plasmid sequence
Borrelia burgdorferi plasmid cp32-6, complete plasmid sequence
Borrelia burgdorferi plasmid cp32-7, complete plasmid sequence
Borrelia burgdorferi plasmid cp32-8, complete plasmid sequence
Borrelia burgdorferi plasmid cp32-9, complete plasmid sequence
Borrelia burgdorferi plasmid cp9, complete plasmid sequence
Borrelia burgdorferi plasmid lp17, complete plasmid sequence
Borrelia burgdorferi plasmid lp21, complete plasmid sequence
Borrelia burgdorferi plasmid lp25, complete plasmid sequence
Borrelia burgdorferi plasmid lp28-1, complete plasmid sequence
Borrelia burgdorferi plasmid lp28-2, complete plasmid sequence
Borrelia burgdorferi plasmid lp28-3, complete plasmid sequence
Borrelia burgdorferi plasmid lp28-4, complete plasmid sequence
Borrelia burgdorferi plasmid lp36, complete plasmid sequence
Borrelia burgdorferi plasmid lp38, complete plasmid sequence
Borrelia burgdorferi plasmid lp5, complete plasmid sequence
Borrelia burgdorferi plasmid lp54, complete plasmid sequence
Borrelia burgdorferi plasmid lp56, complete plasmid sequence
Bradyrhizobium japonicum strain USDA110, complete genome
Brucella melitensis strain 16M chromosome I, complete sequence
Brucella melitensis strain 16M chromosome II, complete sequence
Brucella suis 1330 chromosome I complete genome
Brucella suis 1330 chromosome II complete genome
Buchnera aphidicola (Baizongia pistaciae), complete genome
Buchnera aphidicola str. Sg (Schizaphis graminum) complete genome
Buchnera sp. APS complete genome
Buchnera sp. APS plasmid pLeu DNA, complete sequence
Buchnera sp. APS plasmid pTrp DNA, complete sequence
Campylobacter jejuni complete genome
Caulobacter crescentus complete genome
Chlamydia muridarum plasmid pMoPn, complete sequence
Chlamydia muridarum, complete genome
Chlamydia pneumoniae complete genome
Chlamydia trachomatis complete genome
Chlamydophila caviae GPIC complete genome
Chlamydophila caviae GPIC plasmid pCpGP1, complete sequence
Chlamydophila pneumoniae AR39, complete genome
Chlamydophila pneumoniae J138, complete genome
Chlamydophila pneumoniae TW-183, complete genome
Chlorobium tepidum TLS complete genome
Chromobacterium violaceum ATCC 12472 complete genome
Clostridium acetobutylicum ATCC824 complete genome
Clostridium acetobutylicum megaplasmid pSOL1, complete sequence
Clostridium perfringens 13 DNA, complete genome
Clostridium perfringens plasmid pCP13 DNA, complete sequence
Clostridium tetani E88, complete genome
Corynebacterium efficiens YS-314, complete genome
Coxiella burnetii strain RSA 493, complete genome
Deinococcus radiodurans R1 complete chromosome 1
Deinococcus radiodurans R1 complete chromosome 2
Deinococcus radiodurans R1 megaplasmid MP1, complete plasmid sequence
Deinococcus radiodurans R1 plasmid CP1, complete plasmid sequence
Enterococcus faecalis V583, complete genome
Escherichia coli CFT073 complete genome
Escherichia coli K-12 MG1655 complete genome
Escherichia coli O157:H7, complete genome
Fusobacterium nucleatum subsp. nucleatum ATCC 25586, complete genome
Genomic sequence of a Lyme disease spirochete, Borrelia burgdorferi
Haemophilus ducreyi strain 35000HP complete genome
Haemophilus influenzae Rd complete genome
Halobacterium sp. NRC-1 complete genome
Halobacterium sp. NRC-1 plasmid pNRC100, complete plasmid sequence
Halobacterium sp. NRC-1 plasmid pNRC200 complete genome
Helicobacter hepaticus ATCC 51449 complete genome
Helicobacter pylori 26695 complete genome
Helicobacter pylori, strain J99 complete genome
Lactobacillus plantarum WCFS1, complete genome
Lactococcus lactis subsp. lactis IL1403 complete genome
Leptospira interrogans serovar lai str. 56601 chromosome I, complete sequence
Leptospira interrogans serovar lai str. 56601 chromosome II, complete sequence
Listeria innocua Clip11262, complete genome
Listeria innocua plasmid pLI100, complete sequence
Listeria monocytogenes strain EGD, complete genome
Mesorhizobium loti complete genome, complete sequence
Mesorhizobium loti plasmid pMLa DNA, complete genome, complete sequence
Mesorhizobium loti plasmid pMLb DNA, complete genome, complete sequence
Methanobacterium thermoautotrophicum delta H complete genome
Methanococcus jannaschii complete genome
Methanococcus jannaschii large extra-chromosomal element, complete sequence.~
Methanococcus jannaschii small extra-chromosomal element, complete sequence.~
Methanopyrus kandleri AV19, complete genome
Methanosarcina acetivorans str. C2A, complete genome
Methanosarcina mazei strain Goe1, complete genome
Mycobacterium bovis subsp. bovis AF2122/97 complete genome
Mycobacterium leprae strain TN complete genome
Mycobacterium tuberculosis CDC1551, complete genome
Mycobacterium tuberculosis complete genome
Mycoplasma gallisepticum strain R complete genome
Mycoplasma genitalium G37 complete genome
Mycoplasma penetrans, complete genome
Mycoplasma pneumoniae M129, complete genome
Mycoplasma pulmonis (strain UAB CTIP) complete genome
Neisseria meningitidis serogroup A strain Z2491 complete genome
Neisseria meningitidis serogroup B strain MC58 complete genome
Nitrosomonas europaea ATCC 19718, complete genome
Nostoc sp. PCC 7120 complete genome
Nostoc sp. PCC 7120 plasmid pCC7120alpha DNA, complete genome
Nostoc sp. PCC 7120 plasmid pCC7120beta DNA, complete genome
Nostoc sp. PCC 7120 plasmid pCC7120delta DNA, complete genome
Nostoc sp. PCC 7120 plasmid pCC7120epsilon DNA, complete genome
Nostoc sp. PCC 7120 plasmid pCC7120gamma DNA, complete genome
Nostoc sp. PCC 7120 plasmid pCC7120zeta DNA, complete genome
Oceanobacillus iheyensis, complete genome
Pasteurella multocida PM70 complete genome
Pirellula sp. strain 1 complete genome
Porphyromonas gingivalis W83 complete genome
Prochlorococcus marinus MED4 complete genome
Prochlorococcus marinus MIT9313 complete genome
Prochlorococcus marinus subsp. marinus str. CCMP1375 complete genome
Pseudomonas aeruginosa PA01, complete genome
Pseudomonas putida KT2440 complete genome
Pseudomonas syringae pv. tomato str. DC3000 complete genome
Pyrobaculum aerophilum strain IM2 complete genome
Pyrococcus abyssi complete genome
Pyrococcus furiosus DSM 3638, complete genome
Pyrococcus horikoshii OT3 complete genome
Ralstonia solanacearum GMI1000 chromosome, complete sequence
Ralstonia solanacearum GMI1000 megaplasmid, complete sequence
Rickettsia conorii Malish 7, complete genome
Rickettsia prowazekii strain Madrid E, complete genome
Salmonella enterica serovar Typhi (Salmonella typhi) strain CT18 plasmid pHCM1
Salmonella enterica serovar Typhi (Salmonella typhi) strain CT18 plasmid pHCM2
Salmonella enterica serovar Typhi (Salmonella typhi) strain CT18, complete chromosome
Salmonella enterica subsp. enterica serovar Typhi Ty2, complete genome
Salmonella typhimurium LT2 strain SGSC1412 plasmid pSLT complete sequence
Salmonella typhimurium LT2, complete genome
Shewanella oneidensis MR-1 complete genome
Shewanella oneidensis MR-1 megaplasmid complete sequence
Shigella flexneri 2a str. 2457T complete genome
Shigella flexneri 2a str. 301 complete genome
Sinorhizobium meliloti 1021 complete genomes
Sinorhizobium meliloti plasmid pSymA complete plasmid sequence
Sinorhizobium meliloti plasmid pSymB, complete sequence
Staphylococcus aureus (EMRSA-16) chromosome
Staphylococcus aureus MSSA 8325, genome
Staphylococcus aureus MSSA strain 476
Staphylococcus aureus MW2, complete genome
Staphylococcus aureus plasmid VRSAp, complete sequence
Staphylococcus aureus strain Mu50, complete genome
Staphylococcus aureus strain N315, complete genome
Staphylococcus aureus subsp. aureus N315 plasmid pN315B DNA, complete sequence
Staphylococcus epidermidis ATCC 12228, complete genome
Streptococcus agalactiae 2603, complete genome
Streptococcus agalactiae NEM316, complete genome
Streptococcus mutans UA159 complete genome
Streptococcus pneumoniae R6 complete genome
Streptococcus pneumoniae complete genome
Streptococcus pyogenes MGAS315, complete genome
Streptococcus pyogenes SSI-1, complete genome
Streptococcus pyogenes strain MGAS8232, complete genome
Streptococcus pyogenes strain SF370 serotype M1, complete genome
Streptomyces avermitilis plasmid SAP1 DNA, complete genome
Streptomyces avermitilis, complete genome
Streptomyces coelicolor A3(2), complete genome
Streptomyces coelicolor plasmid SCP1
Streptomyces coelicolor plasmid SCP2
Sulfolobus solfataricus, complete genome
Sulfolobus tokodaii complete genome
Synechococcus sp. WH8102 complete genome
Synechocystis PCC6803 complete genome
Thermoanaerobacter tengcongensis strain MB4T, complete genome
Thermoplasma acidophilum, complete genome
Thermoplasma volcanium, complete genome
Thermosynechococcus elongatus BP-1, complete genome
Thermotoga maritima complete genome
Ti plasmid, complete sequence
Treponema pallidum complete genome
Tropheryma whipplei TW08/27, complete genome
Tropheryma whipplei str. Twist, complete genome
Ureaplasma urealyticum complete genome
Vibrio cholerae chromosome I, complete chromosome
Vibrio cholerae chromosome II, complete chromosome
Vibrio parahaemolyticus RIMD 2210633, chromosome 2, complete sequence
Vibrio parahaemolyticus, chromosome 1, complete sequence
Vibrio vulnificus CMCP6 chromosome I complete sequence
Vibrio vulnificus CMCP6 chromosome II complete sequence
Wigglesworthia brevipalpis, complete genome
Wolinella succinogenes, complete genome
Xanthomonas axonopodis pv. citri str. 306, complete genome
Xanthomonas campestris pv. campestris str. ATCC 33913, complete genome
Xylella fastidiosa Temecula1 plasmid pXFPD1.3, complete sequence
Xylella fastidiosa Temecula1, complete genome
Xylella fastidiosa plasmid pXF1.3, complete sequence
Xylella fastidiosa plasmid pXF51, complete sequence
Xylella fastidiosa, complete genome
Yersinia pestis KIM complete genome
Yersinia pestis plasmid pCD1
Yersinia pestis plasmid pPCP1
Yersinia pestis plasmid pPMT1
Yersinia pestis strain CO92, complete genome
OR paste the raw sequence in the box below
and supply a name for it:
OR provide a sequence by uploading a file
Choose the format of your sequence :
FastA
GenBank
SwissProt
EMBL
PIR
GCG
not sure
Choose the enzyme you want to cut with :
AluI
ApaI
BamHI
BglII
Csp6I
EcoRI
EcoRV
HhaI
HindIII
KpnI
MboI
MboII
MluI
MseI
PstI
PvuI
PvuII
RsaI
Sau3AI
TaqI
Selective base(s)
Maximum fragment size:
AluI
ApaI
BamHI
BglII
Csp6I
EcoRI
EcoRV
HhaI
HindIII
KpnI
MboI
MboII
MluI
MseI
PstI
PvuI
PvuII
RsaI
Sau3AI
TaqI
Selective base(s)
Minimum fragment size:
Optional
Paste start and end points of targets here